GSM2035731: EB cells infected with FUGW-GFP-HOXA7; Homo sapiens; RNA-... - SRA

SRX1526348: GSM2035731: EB cells infected with FUGW-GFP-HOXA7; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 14.7M spots, 735.1M bases, 311.8Mb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq expression data from EB-HSPCs after HOXA7 overexpression

PRJNA308424 • SRP068281 • All experiments • All runs

show Abstracthide Abstract

HOXA7 regulates FL-HSPC self-renewal in vitro and in vivo. We profiled EB-HSPCs after HOXA7 overexpression (EB-HOXA7), or with a control vector (EB-CTR), to assess the gene expression programs regulated by HOXA7. Overall design: CD34+CD38-CD43+CD90+ HSPCs were infected with lentiviral FUGW vector either empty (FUGW-GFP) or encoding HOXA7(FUGW-GFP-HOXA7) protein. Cells were expanded on op9 for 15 days and than sorted for GFP HSPC immunophenotype.

Sample: EB cells infected with FUGW-GFP-HOXA7

SAMN04395238 • SRS1244245 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was purified from sorted cells using RNeasy Mini Kit (QIAGEN), according to manufacturer's instructions. Ovation Rna-seq system System v2 (Nugen), followed by KAPA LTP Library Preparation Kit

Experiment attributes:

GEO Accession: GSM2035731

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 14.7M spots, 735.1M bases, 311.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR3095999	14,701,476	735.1M	311.8Mb	2016-05-17

ID:: 2157619

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